Abstract
In this chapter, we describe the use of recombinant VV to express G alpha subunits efficiently in S49 cyc- cells, a murine lymphoma cell line deficient in endogenous Gs alpha activity. Additional studies have shown that VV infection does not interfere with G-protein-coupled signal transduction events. In particular, the VV-expressed rat Gs alpha subunit is able to mediate efficient receptor-dependent and receptor-independent activation of adenylyl cyclase in cyc- cells. This system should therefore be useful for rapidly assessing the activity of modified or chimeric Gs alpha subunits.
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