Abstract
Publisher Summary This chapter describes the biosynthetic formation of 14 C-labeled C 15 -C 60 polyprenols that may be used as standards, and their separation and chain length determination by High-Performance Liquid Chromatography (HPLC). Phosphorylated polyprenols may also be analyzed in this way when converted to their corresponding alcohols by alkaline phosphatase, hydrolysis, or reduction with LiAIH 4 , because handling of the free alcohols is much easier than of phosphorylated compounds. A number of graphs are obtained, demonstrating the homologous behavior of the compounds. Because of this and the overlapping separation patterns of the different graphs an unequivocal assignment of chain length can be made, although relatively few standards are available. The slight curvature of these graphs is explained by the nonideality of partition on reversed phase columns.
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