Abstract
IN a previous paper1, the incorporation of radioactive inorganic phosphate into human red-cell ghosts was investigated in great detail. About 30 labelled phosphopeptides could be isolated, and their amino-acid patterns were determined. The rate of incorporation and the pH-dependence were also estimated. It was also shown2 that the 32P-incorporating membrane factor could be extracted and purified, and at least some part of the phospholipids also seemed to be metabolically active, since labelled phosphorylethanolamine and phosphorylcholine could be isolated. In addition, it was briefly mentioned that labelled trichloroacetic acid (TCA)-soluble adenosine triphosphate (ATP) seemed to be formed in the labelling process. In the TCA-insoluble material, labelled ultra-violet-absorbing substances were also present.
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