Abstract

Our objective was to characterize epidermal and neuronal interaction in juvenile or adult using a new model of re-innervated epidermis. Human sensory neurons were obtained from iPS cells, their differentiation and functionality were validated by observation of neuronal morphology, neuronal markers expression, and increase of intracellular calcium following lactic acid (LA) stimulation. Human sensory neurons were cultured in presence of epidermis obtained from juvenile and adult donors. The reinnervation of the epidermis was confirmed by PGP9.5 immunostaining revealing the presence of nerve fibres in the epidermis.

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