32. Potential therapeutic in preeclampsia: Effect of resveratrol on endothelial cells incubated with plasma from pregnant before clinical onset of disease

Abstract

Introduction Resveratrol is a compound presented in high quantity in red grapes that contribute to healthy endothelial function. We are exploring an in vitro model of preeclampsia incubating plasma with endothelial cell cultures. In this study we collected samples before clinical onset of preeclampsia that is relevant because a dietary intervention may reduce risk to develop preeclampsia. Objective To verify if resveratrol induces production of NO, improve cell viability and modulate expression of heme oxygenase-1 (HO-1, enzyme inducible by oxidative stress) in endothelial cells incubated with plasma from case and control pregnant. Methods We collected blood at 20–25 weeks of gestation from matched healthy pregnant during all gestation (control, n = 5) and pregnant who subsequently develop preeclampsia (case, n = 5). Plasma was incubated (10% v/v) for 24 h in HUVECs with or not 30 μM trans-resveratrol. Cellular viability was measured using MTT assay, HO-1 by ELISA and nitrite (NO metabolite) by Griess. P value Results We found similar levels of cell viability and nitrite levels between case and control, supplemented or not with resveratrol (all P > 0.05). Concerning HO-1 levels, we found that cultures incubated with plasma from case presented higher levels of HO-1 compared to control. When resveratrol was added, this difference diminished, although plasma from case induce higher HO-1 expression compared to control. Discussion Our study demonstrates that plasma from case induce expression of HO-1 suggesting presence of inducers such as oxidative stress even before clinical onset; and when resveratrol is added reducing of HO-1 expression in case cultures demonstrated lower cell-stress. Our results suggest that a diet rich in resveratrol may help to prevent endothelial injury decrease risk to develop preeclampsia, as supported by literature in cardiovascular diseases. This study was funded by FAPESP (grant #2015/20461-8) and CNPq (grant #2014-5/305587).