32] Interferon induction by polynucleotides, modified polynucleotides, and polycarboxylates

Abstract

Publisher Summary This chapter discusses the procedures for obtaining high interferon yields in primary rabbit kidney (PRK) and human fibroblasts (HF) cells, in response to (I) n . (C) n . The chapter discusses several procedures that are developed to enhance the interferon response of PRK and HF cell cultures to (I) n . (C) n . An appropriate interferon induction scheme has been applied routinely to assess the interferon-inducing potentials of double-stranded RNAs in HF cells and could be readily adjusted to obtain human fibroblast interferon in large quantities. Parenteral administration of (I) n . (C) n results in an appreciable interferon response in most animal species in which (I) n . (C) n has been tested: upon intravenous injection of (I) n . (C) n , peak serum interferon titers will be attained at 2 hours in rabbits, 2-4 hours in mice, 3-6 hours in cats, 8 hours in monkeys, and 12-24 hours in man. As regularly done with (I) n . (C) n , polycarboxylates could also be injected intravenously to elicit an interferon response, and, indeed, interferon has been detected in the serum of humans given single or repeated intravenous doses of 8-16 mg/kg of pyran copolymer.