Abstract

1. 1. Phosphorus-31 nuclear magnetic resonance spectroscopy ( 31P-NMR) was used to examine six species of living adult worms representing the three orders of the Class Oligochaeta. 2. 2. The presence and relative abundance of phosphorus-containing compounds (phosphomonoesters, inorganic phosphate, phosphodiesters, phosphagens, free nucleotide triphosphates, and dinucleotides) were determined and compared between taxa. 3. 3. Our results indicate an evolutionary conservation of phosphagen content (phospholombricine) in both terrestrial and freshwater oligochaetes, a result that contrasts with the evolutionary divergence of phosphagen types in marine polychaete worms.

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