Abstract
The energy status of Romanomermis culicivorax-parasitized and control Culex pipiens larvae was studied in vivo with 31P nuclear magnetic resonance (NMR) at 4–6°C. Peak assignments were made for inorganic phosphorus, phosphoarginine, AMP, ADP, and ATP. The mean ATP ADP ratios were 4.45 for control and 4.02 and 2.94 for parasitized mosquito larvae with 1.3 and 2.3 parasites per host, respectively. The ATP ADP ratios for control mosquito larvae reared with a pH of 7 and 4.5 were not significantly different. R. culicivorax parasitism had no significant effect on the ATP level in whole mosquito larvae. The mean ATP concentrations were 9.86 and 11.64 nmol/mg dry weight mosquito for control and parasitized larvae, respectively. R. culicivorax postparasites (10 days post-infection) were shown to have a very weak 31P NMR signal and it was concluded that the parasites contribute little to the NMR spectra of infected mosquito larvae. Enzymatic analysis of perchloric acid-extracted control mosquito larvae yielded 9.3 nmol ATP/mg dry wt and 2.6 nmol ATP/mg dry weight for R. culicivorax postparasites at 10 days post-infection.
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