Abstract
Abstract Phosphorus-31 nuclear spin-lattice relaxation times and nuclear Overhauser enhancement upon nonselective proton saturation were measured on polyriboadenylic acid, polyribocytidylic acid, and polyribouridylic acid. A method is described to separate dipolar contribution from the contribution of chemical shift anisotropy for the phosphorus relaxation by the combined use of T1 and NOE data. Chemical shift anisotropies (δ⁄⁄−δ⊥) of phosphorus in these polynucleotides in solution are estimated to be in the range 120–165 ppm. The molecular motion of the phosphorus moiety becomes faster in the order, poly(A)<poly(C)<poly(U), the thermal activation processes being characterized by single activation energies, i.e., 5.1, 5.3–6.0, and 7.2–8.1 kcal/mol for poly(U), poly(C), and poly(A), respectively.
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