Abstract
Micropropagation of herbaceous peony (Paeonia) cultivars and hybrids (Paula Fay, Cytherea, Prairie Moon, and Sarah Bernhardt) was investigated. Root clumps were removed from the field in February and forced in the greenhouse. Explants were excised buds from the crown area. Culture contamination levels were reduced by selection of crown buds prior to budbreak and disinfestation using combination treatments of 20 min with 5% potassium iodide followed by 10 min with 10% bleach. Genotypes responded differently to adventitious multiplication (Stage II) and tuberous root formation (Stage III) in Murashige and Skoog basal medium supplemented with 6-benzylaminopurine and gibberellic acid (Stage II) or indolebutyric acid (Stage III). Transfer to the greenhouse was accomplished (Stage IV) with a limited number of plants.
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