Experimental Hematology | VOL. 88
Read

3146 – INVESTIGATING THE UBIQUITIN E3 LIGASE, FBXO21, IN REGULATION OF HEMATOPOIETIC STEM CELLS

Publication Date Aug 1, 2020

Abstract

The lifetime process of hematopoiesis is highly regulated through molecular pathways directing hematopoietic cell fate decisions such as self-renewal and differentiation in the hematopoietic stem and progenitor cell (HSPC) population. The ubiquitin proteasome system (UPS) regulates many cellular processes through controlling protein levels by tagging them with polyubiquitin chains and promoting their degradation through the proteasome. The substrate recognition component of the UPS is the ubiquitin E3 ligase. Through investigating a specific family of ubiquitin E3 ligases, the Fbox family of proteins, which contains about 70 E3 ligases, we discovered that Fbxo21 was highly expressed in the HSPC population. Fbxo21 was more highly expressed in the HSPC population when compared to other Fbox genes. In addition, Fbxo21 expression was primarily in immature hematopoietic populations, whereas, more mature hematopoietic populations had limited expression. Western blot and qRT-PCR analysis confirmed high expression of FBXO21 in HSPC, and revealed low to no expression in mature myeloid populations. To determine the role of FBXO21 on HSPC maintenance, self-renewal, and differentiation, we generated shRNAs against Fbxo21. We found the silencing of Fbxo21 in HSPC leads to a loss of colonies and differentiation towards the mature myeloid lineage. These findings lead to the hypothesis that the ubiquitin E3 ligase FBXO21, and its substrates regulate hematopoietic stem cell maintenance and loss promotes myeloid differentiation. Deciphering t...

Concepts

Hematopoietic Stem And Progenitor Cell Hematopoietic Stem And Progenitor Cell Population Family Of Ubiquitin E3 Ligases Fbox Family Of Proteins E3 Ligase Ubiquitin Proteasome System Hematopoietic Stem Cell Maintenance Hematopoietic Stem Cell Hematopoietic Cell Fbox Genes

Round-ups are the summaries of handpicked papers around trending topics published every week. These would enable you to scan through a collection of papers and decide if the paper is relevant to you before actually investing time into reading it.

Climate change Research Articles published between Nov 14, 2022 to Nov 20, 2022

R DiscoveryNov 21, 2022
R DiscoveryArticles Included:  2

The world needs to move quickly towards sustainability that should characterize all production and consumption patterns. We use cookies to improve you...

Read More

Coronavirus Pandemic

You can also read COVID related content on R COVID-19

R ProductsCOVID-19

ONE PROBLEM . ONE PURPOSE . ONE PLACE

Creating the world’s largest AI-driven & human-curated collection of research, news, expert recommendations and educational resources on COVID-19

COVID-19 Dashboard

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on “as is” basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The Copyright Law.