Abstract

Abstract Forthcoming revisions to Canadian Transport regulations indicate un-weaned and weaned calves can be transported a maximum of 12 and 36 h, respectively, before an 8 h rest is required. The aim of this study was to assess the effect of rest duration after 12 and 36 h of transport on physiological and behavioral indicators of welfare in 7–8 mo old beef calves. Three hundred and twenty weaned calves (258 ± 23.9 kg BW) were randomly assigned to a 2 × 4 factorial design: 12 and 36 h of transport; and 0 (R0), 4 (R4), 8 (R8), and 12 (R12) h of rest. After the resting period animals were transported for an additional 4 h. A subset of 12 animals/treatment were sampled for non-esterified fatty acids (NEFA), haptoglobin and lactate concentrations prior to, and after the first and the 4 h transport, and 7 h, 2 and 28 d after the 4 h transport. Standing and lying behavior was assessed for 14 d after transport. Data was analyzed using the GLIMMIX procedure of SAS, where transport, and time nested within rest period were fixed effects and animal was a random effect. NEFA concentrations were greater (P < 0.01) in 12-R4 than 12-R8 and 12-R12 calves, while 36-R0 calves had greater (P ≤ 0.05) NEFA concentrations than 36-R4, 36-R8 and 36-R12 calves after the 4 h transport. Haptoglobin concentrations were greater (P < 0.01) in 36 than 12 h calves. No differences (P > 0.10) were observed for lactate. The day after transport, 36-R8 calves spent more (P < 0.01) time lying than 12-R8 calves. Overall, physiological indicators were greater in calves transported for 36 than 12 h, while no differences were observed between rest stops with the exception of NEFA, where overall concentrations were greater after shorter than longer rest periods.

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