Abstract
Publisher Summary This chapter discusses the quantitative micro complement fixation. Quantitative micro complement fixation is a most sensitive serologic method for detection of conformational changes in macromolecules. Denaturation of DNA and polyribonucleotides, altered conformation of lactic dehydrogenases, hemoglobin, myoglobin, collagen, lysozyme, aspartate transcarbamylase, pepsinogen and pepsin, carboxypeptidase, and S-100 brain protein have been detected and quantified by micro complement fixation. In addition, the extreme sensitivity of the method has been exploited to measure the molecular changes induced by evolutionary processes. This chapter describes the use of micro complement fixation to measure the rates of dissociation and the equilibrium constants for dissociation of pig liver carboxylesterase as a function of pH and salt concentration. The pig liver carboxylesterase preparation and some of its physical properties as described by Barker and Jencks are discussed in this chapter. Estimation of native carboxylesterase molecules is discussed. Equilibrium constants, as measured by complement (C) fixation, for dissociation of pig liver carboxylesterasc as a function of pH are illustrated. Determination of equilibrium constants is also discussed in this chapter in detail.
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