Abstract
Aminosilanized micro patterns were transferred on mica substrate using PDMS soft stamp fabricated by utilizing anisotropic etched Si substrate as a mold and proteoglycan molecular immobilization were investigated. Two types of parting agents were investigated to improve detachability of PDMS stamp from Si mold, and pillar arrays with height of 5-6μm and 10μm x 10μm wide were formed. PDMS pillar-arrays with ultra-smooth surface were formed. Residual parting agents on PDMS stamp surface after peeling off, were removed by repetitive dummy stamps on mica substrate. After PDMS stamp was dipped into APTES solution, followed by nitrogen blow drying, APTES molecules on PDMS stamp were transferred on mica substrate with over 10g of load. PG solution was deposited on APTES transferred mica substrate. PG molecules were immobilized inside and outside APTES transferred area. PG molecules with widely spread GAG molecules were successfully immobilized inside the pattern due to APTES treated effect whereas PG molecules without GAG spreading were also deposited outside the pattern.
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