Abstract

Spinach chloroplasts are spread at a heptane/water interface. With a novel capacitive electrode [H.-W. Trissl Biochim. Biophys. Acta, 595 (1979) 82] the changes of the interfacial potential induced by single laser flashes are investigated. The state of the interfacial thylakoid layer is characterized by measurements of the interfacial tension, the fluorescence induction curve and the comparison of the properties of the interfacial potential change with those known from the field-indicating absorption changes of chloroplast suspensions. The following results are obtained: 1. The chloroplasts spread at the interface form a thin layer with asymmetrical orientation. The structural state of this layer is discussed. 2. From the difference between the fluorescence induction curves measured from the interfacial layer and that from a chloroplast suspension, it is concluded that the electron transport from photosystem II into the plastoquinone pool is partially inhibited in chloroplasts spread at the interface.

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