307 Poster - Application of culture-based assays to human malignant breast tissue can infer tumour microbiota composition

Abstract

High-throughput 16S rRNA sequencing (HTS) has recently been used to describe the microbial communities of in-vivo compartments that were up until that point described as “sterile.” Among these is the human breast, the microbiome of which was described by our group in patients with and without breast cancer. The impression of the healthy and malignant mammary microbiome to date has been informed primarily by 16S metabarcoding data, and only sparingly by culture-based assays, which may lead to an imbalanced view of the tumour microbiota.