304 Tonicity signals drive and anti-inflammatory Th17 cell properties

Abstract

304 Tonicity signals drive and anti-inflammatory Th17 cell properties J Matthias, R Noster, M Poltorak, D Soll and C Zielinski 1 Technical University of Munich, Munich, Germany, 2 Technical University of Munich, Munich, Germany and 3 Charite, Berlin, Germany Sodium chloride has recently been demonstrated to strongly enhance the generation of Th17 cells in both mouse and man, which provided mechanistic evidence for the epidemiological correlation of enhanced dietary salt intake and the increasing incidence of autoimmune diseases. Our data support the Th17 cell promoting effect of sodium chloride on human Th17 cell priming. However, Th17 cells acquired anti-inflammatory properties upon stimulation in sodium chloride enriched micromilieus. In addition, sodium chloride also strongly suppressed cytotoxic functions of human T cells. Together, our data suggest that despite the reported association of salt with induction of pathogenic Th17 cells, sodium chloride instead is a potent inducer of the anti-inflammatory Th17 cell subset and also paralyzes cytotoxic T cell properties. Therefore, our data suggest that reduction of sodium chloride levels by either dietary restriction or specific targeting of downstream signaling events could counteract T cell immunoparalysis and exhaustion in settings of chronic inflammatory skin diseases or malignancies. 305 Heat shock protein 60 & 70 expression in psoriasis vulgaris before and after NB-UVB therapy A Kotb Dermatology Research, National Research Center, Cairo, Egypt Psoriasis is a common chronic, relapsing, non-infectious inflammatory skin disease. Heat shock proteins (Hsps) play an important role in essential functions in cells under physiological conditions and play a major role in protecting cells against damage in stressful conditions & they appeared in some studies to play an important role in pathogenesis of many inflammatory skin conditions including psoriasis, atopic dermatitis and systemic lupus erythrematoses. The aim was to detect the expression of HSP60 & HSP70 in psoriasis and its correlation to the disease severity and to review potential role of HSP60 & HSP70 in pathogenesis and therapy of psoriasis. Patients with Psoriasis Vulgaris were treated with NB-UVB & the skin biopsies were taken before & after therapy. The immunoreactivity of HSP60 & HSP70 was detected using IRIDI score. It was confirmed that HSP60 & HSP70 was expressed in psoriatic lesions and their expression is increased with the disease severity but not related to either age or sex & it decreased markedly after exposure to NB-UVB twice weekly for 12 weeks. In conclusion, HSP60 & HSP70 play a role in the pathogenesis of psoriasis & both can be used as a target for therapy.