昭和30年度分離赤痢菌に関する2 3の知見について(第3報)

Abstract

Twelve strains of Coli bacilli showing paraagglutination by Shigella antisera were isolated from a family of five persons of whom three dysenteric patients. The strains were studied on clinical and serological points of view with the following results:1. All members of the Coli group strains studied were found to be related with Shigella groups in that these two groups of bacilli were isolated from the faces of the patients on the same culture plates or some of the microbes were found before or after excretion of Shigella bacilli.2. Biological characters of these Coli strains were not uniform. Most of these strains belong to Esherichia Coli type 1 on the test of IMVIC system. They were of Gram stain negative and no motility. These strains produce indol and some of them utilized mannitol.3. All these strains were found to be related with Shigella bacilli in agglutination. In some of the strains their agglutinability was low, while others were highly reactive and extensive just like Shigella Flexneri 2a. They were agglutinated by Shigella serotype II and group 4 antisera. O-antigen suspentions prepared with heated cultures of the employed strains were usually agglutinated with higher titers in some Shigella flexneri antiserum than the titers ovserved in nonheated bacilli suspentions.4. The agglutinability of these strains to Shigella antisera were markedly diminished or disappeared after several inoculation, e. g., after the 4th subculture some of them lost their agglutinability to Shigella sera and a few became to spontanously agglutinable.5. The results of agglutination test by E. Coli type specific antisera indicated that the Oantigens of two of these cultures were found to be O-126, four of them to be O-26 (one is uncertain !), and three of them to be O-25 (but two are uncertain !).