30-100 kHz, 2 ns passively Q-switched laser for fast and efficient photoacoustic microscopy.

Abstract

Actively Q-switched (AQS) fiber laser and solid-state laser (SSL) are widely used for photoacoustic microscopy (PAM). In contrast, passively Q-switched (PQS) SSL not only maintains most of the merits of AQS lasers, but also exhibits unique advantages, including the pulse width (PW), pulse repetition rate (PRR) tunability, wavelength, compactness, and cost. These advantages all benefit the PAM. However, there are few reports demonstrating the performance of PQS-SSL on PA imaging. Here, we demonstrate a compact PQS-SSL for fast and efficient PA imaging. The laser uniquely maintains a constant PW (~2 ns) and pulse energy (~3 μJ) during the PRR variation (30-100 kHz), which is valuable for preserving a stabilized imaging performance at different scanning rates. The PA imaging performance is compared by a resolution target and showcased by whole-body scanning of an embryonic zebrafish in vivo. The performance indicates that PQS-SSL is a promising candidate for PAM.