300 Using single cell technique to reveal the expression landscape of lncRNAs in psoriasis

Abstract

Long-noncoding RNAs (lncRNAs) have been shown to regulate epidermal differentiation in skin, and bulk RNA-seq data have illustrated the tissue and cell type specificity for skin-expressing lncRNAs. In this study, we assessed the ability to use single cell RNA-seq (scRNA-seq) obtained from 9 psoriasis lesional skin (PP) and 6 uninvolved skin (PN) samples to characterize the expression profiles for lncRNAs in cell-level resolution. Despite the limitation of scRNA-seq in detecting low-expressing transcripts, we identified 4,785 expressing lncRNAs (using threshold of >=2 reads in at least one cell) in majority of the cells.