300 - Conditional deletion of thioredoxin 1 in the adult mouse causes multiple tissue failures and early death

Abstract

Although thioredoxin 1 (Trx1) is ubiquitously expressed, necessary for development, and has modified activity during pathogenesis of numerous oxidative diseases, very little is known about the role of this oxidoreductase in maintenance of adult tissue function. To determine physiological functions of Trx1, we developed doxycycline-inducible Trx1 conditional knockout (KO) mice. Administration of doxycycline to adult mice resulted in death or moribund conditions requiring euthanasia in 13-25 days post treatment. By treatment day 10, KO mice lost 4.2±1.5% and control mice gained 5.2±1.9% of initial body weight (P