在3.0 T 磁場下建立口腔癌之氫-1核種磁振頻譜技術

Abstract

Abstract Purpose The aim of this research is to establish an in vivo H-1 MR Spectroscopy (MRS) technique in the position of the oral cavity in routine clinical practice at 3.0 T. Several localization techniques and MRS parameters will be evaluated in order to select the most suitable method to collect MR signal from a thin layer of oral cancer. This technique will help to expand the MR spectroscopy research in distinguish the benign/ malignant tumor and monitor the result of tumor treatment in routine clinical practice. Material and method The experiments were performed on a GE Signa 3.0 T system equipped with the standard MRS analysis software (SAGE 7.3). The standard H-1 head coil was used for both imaging and spectroscopy. The optimize localization technique and parameters were first tested with the known concentration phantom and the normal from the oral cavity to confirm the adequacy of the technique. This technique was further applied to the tumor of VX2 animals as well as cancer patients of oral cavity to demonstrate the feasibility of detecting the existence of choline in malignant tumor. The H-1 MR spectroscopic imaging data is collected by Single-voxel and 1D chemical shift imaging (CSI). After acquisition, the raw data was zero filled once, apodized with a 5 Hz Gaussian filter, Fourier transform, phase correction and baseline correction. Marquardt curve fitting with Gaussian line shape was used to calculate the area under the peak. Results Establishes the single-voxel technology and the 1D-CSI technology by using the standard phantom, simultaneously appraises its system stability. In the short-term stability, the coefficient of variance (CV) of the detected Cho and Cr from 1D-CSI technique were 11% and 12% ,and from single voxel technique were 8% and 7%. In the long -term stability, the CV of the detected Cho and Cr from 1D-CSI were 16% and 17%. Conclusions This research has established an in vivo H-1 MRS technique at 3.0 T. The technique will help to expand the MR spectroscopy research in the clinical oral cavity cancer in distinguishing the benign/ malignant tumor and monitoring the result of tumor treatment in routine clinical practice.