Abstract
Introduction Endothelial colony-forming cells (ECFCs) hold great cytotherapeutic potential for ischaemic disease. Interestingly, recent evidence supports a key role for NADPH oxidases in underlying angiogenic processes of these and other endothelial cells. Aims To study the effects of Nox NADPH oxidases on pro-angiogenic function of ECFCs. Methods Human ECFCs isolated from umbilical cord blood were treated with pro-oxidant PMA and assessed in vitro , both basally and after siRNA knockdown of Nox4, a key endothelial isoform, alongside primary mature human aortic endothelial cells (HAoECs). Results PMA (500 nM for 8 h) increased cell migration (control 18.6 ± 2.8, PMA 32.7 ± 6.6% closure; n = 6, P Conclusion ECFC migration is enhanced by low concentrations of superoxide, and to a greater extent as compared to mature endothelial cells, and appears to be at least partly dependent upon Nox4 NADPH oxidase. These findings may have significant implications for potential ECFC-based therapies for ischaemic disease, which is associated with an oxidative microenvironment.
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