30 ALPHA1D-ADRENERGIC RECEPTOR PLAYS IMPORTANT ROLES FOR COLD STRESS-INDUCED DETRUSOR OVERACTIVITY IN RATS WITH BLADDER OUTLET OBSTRUCTION

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research (1)1 Apr 201330 ALPHA1D-ADRENERGIC RECEPTOR PLAYS IMPORTANT ROLES FOR COLD STRESS-INDUCED DETRUSOR OVERACTIVITY IN RATS WITH BLADDER OUTLET OBSTRUCTION Takahiro Yamagishi, Osamu Ishizuka, Tetsuya Imamura, Wataru Noguchi, Hitoshi Yokoyama, Teruyuki Ogawa, Yoshiki Kurizaki, Osamu Nishizawa, and Karl-Erik Andersson Takahiro YamagishiTakahiro Yamagishi Matsumoto, Japan More articles by this author , Osamu IshizukaOsamu Ishizuka Matsumoto, Japan More articles by this author , Tetsuya ImamuraTetsuya Imamura Matsumoto, Japan More articles by this author , Wataru NoguchiWataru Noguchi Matsumoto, Japan More articles by this author , Hitoshi YokoyamaHitoshi Yokoyama Matsumoto, Japan More articles by this author , Teruyuki OgawaTeruyuki Ogawa Matsumoto, Japan More articles by this author , Yoshiki KurizakiYoshiki Kurizaki Matsumoto, Japan More articles by this author , Osamu NishizawaOsamu Nishizawa Matsumoto, Japan More articles by this author , and Karl-Erik AnderssonKarl-Erik Andersson Winston Salem, NC More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1405AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES In patients with bladder outlet obstruction, cold stress that is suddenly drop or continuously exposure to low environmental temperature exacerbates lower urinary tract symptoms such as urinary urgency and frequency. We previously reported that alpha 1 adrenoceptors play the important roles for cold stress-induced detrusor overactivity in normal rats (Neurourol Urodyn. 2008). In this study, we determined if alpha1D-adrenergic receptor antagonist, naftopidil, could improve cold stress-induced detrusor overactivity in rat with bladder outlet obstruction. METHODS Twenty-six 10-weeks female Sprague-Dawley rats were divided into two groups: naftopidil and vehicle-treated rats, and then their urethra were litigated. After 4 weeks of keeping, the rats were cannulated for cystometric investigations. Micturition patterns of the cannulated conscious rats were recorded at room temperature (RT, 27 ± 2°C) for 20 min. The rats were intravenously administered with 0.3 mg/kg naftopidil or vehicle. Five min later, they were smoothly transferred to a low temperature (LT, 4 ± 2°C), and then the micturition patterns were again recorded for 40 min. The rats that showed 2 to 5 ml bladder capacity under room temperature were estimated. RESULTS Under RT, there were no differences between vehicle-treated control and naftopidil-treated rats. After transferring to LT, voiding interval and bladder capacity of the control rats were significantly decreased. In the naftopidil-treated rats, the decreases of voiding interval and bladder capacity under LT period were significantly inhibited compared to the control rats. CONCLUSIONS This study showed that alpha1D-adrenergic receptors play important roles for detrusor overactivity exacerbated by cold stress in rats with bladder outlet obstruction. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e12 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Takahiro Yamagishi Matsumoto, Japan More articles by this author Osamu Ishizuka Matsumoto, Japan More articles by this author Tetsuya Imamura Matsumoto, Japan More articles by this author Wataru Noguchi Matsumoto, Japan More articles by this author Hitoshi Yokoyama Matsumoto, Japan More articles by this author Teruyuki Ogawa Matsumoto, Japan More articles by this author Yoshiki Kurizaki Matsumoto, Japan More articles by this author Osamu Nishizawa Matsumoto, Japan More articles by this author Karl-Erik Andersson Winston Salem, NC More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...