Abstract
Publisher Summary This chapter provides information on the ubiquinone oxidoreductase (complex I) enzyme. Complex I is the segment of the respiratory chain responsible for electron transfer from NADH (and NADPH) to ubiquinone. The composition of complex I in terms of flavin (FMN), nonheme iron, acid-labile sulfide, ubiquinone, and phospholipids is also given in the chapter. Complex I is highly specific for NADH and ubiquinone isoprenologs as substrates. Special conditions are required to demonstrate satisfactory NADH oxidation by the higher isoprenologs. The preparation can oxidize NADPH at a slow rate and is capable of transhydrogenation from NADH and NADPH to NAD. Complex I catalyze the reduction of ubiquinones and ferricyanide by NADH. NADH-ubiquinone reductase activity can be assayed spectrophotometrically by monitoring the oxidation of NADH at 340 nm.
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