3-Nitropropionic acid depresses spinal reflexes involving GABAergic and glycinergic transmission in neonatal rat spinal cord in vitro

Abstract

Aims 3-Nitropropionic acid (3-NPA) is a naturally occurring fungal toxin that leads to ATP-depletion by inhibiting mitochondrial succinate dehydrogenase and produces chemical anoxia. The present study was conducted to identify the involvement of inhibitory system in 3-NPA-induced depression of spinal reflexes. Methods The monosynaptic (MSR) and polysynaptic reflex (PSR) potentials were recorded at ventral root by stimulating the corresponding dorsal root in hemisected (sagitally) spinal cord from 4–8 day old rats. Effect of 3-NPA in the absence and presence of antagonists was evaluated on the reflexes. Key findings Superfusion of 3-NPA (3.4 mM) depressed the reflexes in a time-dependent manner abolishing them by 35 min. The T-50 values were around 18 and 16 min for MSR and PSR, respectively. An NMDA receptor antagonist, DL-2-amino-5-phosphonovaleric acid (10 μM) failed to block the 3-NPA (3.4 mM)-induced depression of reflexes. Superfusion of bicuculline (GABA A receptor antagonist; 1 μM), or strychnine (glycine A receptor antagonist; 1 μM) antagonized the 3-NPA-induced depression of reflexes significantly. The T-50 values were 26 and 30 min in bicuculline and strychnine pretreated groups, respectively and were significantly greater than 3-NPA only group. Significance The results indicate that 3-NPA-induced depression of spinal reflexes is partially mediated by GABAergic and glycinergic inhibitory transmission.