Abstract
Lysophosphatidic acid (LPA) is a commonly existing bioactive lipid with multiple functions. Its functions are known to be mediated via its cognate G-protein-coupled receptors. It involves in many biological activities, including cell growth, differentiation, survival, apoptotic, and so on. LPA receptor 3 (EDG7) is a member of endothelial cell differentiation gene (EDG) family, which expresses in blood endothelial cells and is expected to participate in angiogenesis. However, the lpa3-null mice fail to show notable defects in angiogenesis. To further explore the role of LPA3, I have cloned an lpa3 gene with its complete sequence from zebrafish. Expression analysis by RT-PCR showed that zebrafish lpa3 (zlpa3) ubiquitously expresses in early embryos and adult tissues. By whole-mount in situ hybridization, it revealed that zlpa3 expresses abundantly in the head and regions surrounding notochord. Using the zlpa3 anti-sense morpholino oligonucleotides (zlpa3-MO), I observed that knockdown of zlpa3 resulted in defects in heart circulation, blood cell formation and neurogenesis. The zlpa3-MO resultant defects are specific since they could be rescued by ectopic expression of zlpa3. In conclusion, this study suggests that LPA3 plays an important role in regulating circulation, hematopoiesis and neurogenesis.
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