Abstract
The calculation of 3-D images of paracrystalline objects is often done using methods appropriate to 2-D crystals. However, this method leads to a loss of information about the structural variations within the paracrystal. Structural variations are often referred to as noise but may represent important features. In our own work with 3-D imaging of sectioned insect flight muscle (IFM) structural variations represent the adjustment in crossbridge form imposed by the filament lattice. In order to complement our averaged 3-D images obtained by 2-D crystal methods, we have experimented with performing essentially the same procedures using all the data, both sampled and continuous, to generate the 3-D image. While the method embodies no new principles, the combination of protocols appears to be unique.The procedure uses the following steps: (1) A uniaxis tilt series ranging to ±75° is recorded. The increment between tilt angles decreases as tilt angle increases and ranges from ˜8° at low angles to ˜2° at high tilts.
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