Abstract
Investigations were carried out on 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX), a potent direct-acting bacterial mutagen found in chlorinated tap water, for the potential to induce 6-thioguanine (6-TG) resistant mutations in cultured Chinese hamster V79 cells. The mutagenicity of MX was manifested when cells were treated with MX in Hanks balanced salt solution, but not in serum-free Eagle's minimal essential medium. A 12-fold higher mutation frequency over control was obtained at 12.5 micrograms/ml MX. In addition, the inhibitory effect of MX on metabolic cooperation between cocultivated 6-TG- sensitive (6-TGs) and -resistant (6-TGr) V79 cells was investigated. Recovery of 6-TGr cells significantly increased at 0.8-2.0 micrograms/ml MX due to impaired intercellular communication with 6-TGs cells. MX is believed to exert tumor-promoting and mutagenic activity in mammalian cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
