Abstract
This chapter discusses various automated electron spin resonance free radical detector assays for antioxidant activity in natural extracts. There is now increasing interest in the antioxidant activity of phytochemicals present in the diet, in health food supplements (neutraceuticals), and in topical preparations for protection of the skin (cosmaceuticals) from environmental exposure. A simple and rapid estimation of hydroxyl and superoxide anion radical scavenging activities by aqueous extract from natural sources can be made using a new computerized JEOL ESR system. Treatment of extracts with ascorbate oxidase reveals that in some cases the presence of vitamin C partially accounts for hydroxyl and superoxide anion radical scavenging activities. Treatments with centrifuge-type filters divide antioxidant activities in a sample to low molecular weight materials and high molecular materials (such as enzymes), and heat treatment also shows the contribution of heat-inactivated components to antioxidant activities in a sample.
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