Abstract
In this paper, the characterization of cellular mechanics of W303 yeast cells was conducted using nanoneedles inside an Environmental-SEM (ESEM). Three types of nanoneedles were used in our experiments, i.e. titanium coated silicon (Si-Ti) and two types of tungsten (W) nanoneedles. The Si-Ti nanoneedle was fabricated using 2 N/m spring constant cantilevers while the W nanoneedles were fabricated using 0.09 N/m and 2 N/m spring constant cantilevers (W_<0.09> and W_2 nanoneedles). The Si-Ti, W_<0.09> and W_2 nanoneedles are suitable to be used for local stiffness characterization of single cells. This capability can be used in future for fast disease detection since disease cells may show different cell mechanics properties compared to the normal cells. The Si-Ti and W_2 nanoneedles can penetrate the cell without cell bursting and this could be important in single cell surgery in future to eliminate cell damage.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: The Proceedings of JSME annual Conference on Robotics and Mechatronics (Robomec)
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
