Abstract
DNA damage detected by the comet assay (single cell gel electrophoresis) and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) formation in DNA in the bone marrow has been studied in groups of 6 male Wistar rats treated with a single i.p. injection of the carcinogen 2-nitropropane (2-NP, 100 mg/kg body weight) or vehicle. Twenty-four hours after 2-NP the average tail length in the comet assay in bone marrow cells was increased from 1.46±0.27 to 9.61±1.56 μm (mean±SD, p<0.01), and 8-oxodG levels in the DNA were increased from 1.04±0.50 to 5.14±2.42 per 10 5 dG ( p<0.01). There was a close correlation between the comet tail length and the 8-oxodG level ( r=0.89, p<0.05). The results indicate that 2-NP inflicts DNA damage in the bone marrow cells and thus could be leukemogenic.
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