Abstract
We aim to validate the theoretical frameworks independently established by Lizzi et al. and Insana et al. relating spectral parameters derived from ultrasonic (US) radio-frequency (RF) echo signals to scatterer properties. A 35-MHz transducer was used to acquire US RF echo signals from isolated, living, uveal melanoma cells suspended in a liquid culture medium. Spectral parameters for these cells were computed from the US RF data using standard Fourier methods and then compared to parameter values predicted by the Lizzi theoretical framework. Initial results show good agreement between experiment and theory, and this study shows promise for extending the framework to include scattering from non-isotropic and densely packed scatterers and for reducing the dependence of these frameworks on numerous key assumptions
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