Abstract
We automated removal of the swine oocyte zona pellucida using a 2DOF magnetically driven microtool (MMT) on a microfluidic chip. Multiple oocytes can be manipulated simultaneously highly stably, providing strong advantages over conventional manual manipulation using pipetting. We propose automating this process by activating a soft polymer microtool on a disposable microfluidic chip to stably mass-produce peeled oocytes - a breakthrough in high throughput and effective oocyte manipulation in cloning and fertility treatment.
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