Abstract
A poly-(l)-lysine (PLL) film cast on an ATR plate (ATR: attenuated total reflection) and hydrated with D2O (80% relative humidity, 28 °C) was exposed to a periodic temperature variation of ΔT/2 = +/−2 °C at the mean value Tmean = 28 °C. Under these conditions the well known structural change from α-helix to β-sheet occurred reversibly in a time range of minutes. A T-stimulation period of τm = 14.7 min turned out to be adequate to induce significant phase shifts between the boundary conformations α-helix/β-sheet and the conformations of transient species. So far, little experimental information was available on these transients. However, phase-resolved, temperature-modulated excitation infrared (T-MEIR) spectroscopy enabled enhanced experimental separation of strongly overlapped bands. T-MEIR spectra were obtained by phase sensitive detection (PSD) of periodically induced changes in the infrared spectrum of PLL. They feature dynamic changes of the components of the amide I‘ and amide II‘ bands, which are ...
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