Abstract
We report the facile and economic preparation of two-dimensional (2D) and 0D MoSe2 nanostructures based on systematic and non-toxic top-down strategies. We demonstrate the intrinsic peroxidase-like activity of these MoSe2 nanostructures. The catalytic processes begin with facilitated decomposition of H2O2 by using MoSe2 nanostructures as peroxidase mimetics. In turn, a large amount of generated radicals oxidizes 3,3,5,5-tetramethylbenzidine (TMB) to produce a visible color reaction. The enzymatic kinetics of our MoSe2 nanostructures complies with typical Michaelis–Menten theory. Catalytic kinetics study reveals a ping–pong mechanism. Moreover, the primary radical responsible for the oxidation of TMB was identified to be Ȯ2− by active species-trapping experiments. Based on the peroxidase mimicking property, we developed a new colorimetric method for H2O2 detection by using 2D and 0D MoSe2 nanostructures. It is shown that the colorimetric sensing capability of our MoSe2 catalysts is comparable to other 2D materials-based colorimetric platforms. For instance, the linear range of H2O2 detection is between 10 and 250 μM by using 2D functionalized MoSe2 nanosheets as an artificial enzyme. Our work develops a systematic approach to use 2D materials to construct novel enzyme-free mimetic for a visual assay of H2O2, which has promising prospects in medical diagnosis and food security monitoring.
Highlights
The development of convenient and sensitive detection of hydrogen peroxide (H2O2) is in high demand in the fields of food security, environmental monitoring and biochemical analysis
Peroxidase-Like Activities and Steady-State Kinetic Assay We evaluated the peroxidase-like activity of MoSe2 quantum dot (QD) by using the catalytic oxidation of TMB
The characteristic excitation-dependent PL of the MoSe2 QDs can be attributed to the polydispersity of the synthesized QDs
Summary
The development of convenient and sensitive detection of hydrogen peroxide (H2O2) is in high demand in the fields of food security, environmental monitoring and biochemical analysis. Various techniques for H2O2 determination have been explored, such as fluorometry [3,4], cellular imaging [5], electrochemistry [6,7], and the colorimetric method [8,9] Among these approaches, the colorimetric method has drawn a lot of attention due to its convenient operation, visibility, facile miniaturization, and low cost [10,11]. The colorimetric method has drawn a lot of attention due to its convenient operation, visibility, facile miniaturization, and low cost [10,11] In this respect, natural enzymes were extensively used for the detection of H2O2 due to its catalysis capability under mild conditions. Enormous progress has been made, the discovery and development of novel promising artificial peroxidase mimics is still in urgent need
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