2-D coupled computational model of biological cell proliferation and nutrient delivery in a perfusion bioreactor

Abstract

Tissue engineering aims to regenerate, repair or replace organs or tissues which have become defective due to trauma, disease or age related degeneration. This engineering may take place within the patient’s body or tissue can be regenerated in a bioreactor for later implantation into the patient. Regeneration of soft tissue is one of the most demanding applications of tissue engineering. Producing proper nutrient supply, uniform cell distribution and high cell density are the important challenges. Many experimental models exist for tissue growth in a bioreactor. It is important to put experiments into a theoretical framework. Mathematical modelling in terms of physical and biochemical mechanisms is the best tool to understand experimental results.In this work a mathematical model of convective and diffusive transport of nutrients and cell evolution in a perfusion bioreactor is developed. A cell-seeded porous scaffold is placed in a perfusion bioreactor and fluid delivers the nutrients to the cells for their growth. The model describes the key features of the tissue engineering processes which includes the interaction between the cell growth, variation of material permeability due to cell proliferation, flow of fluid through the material and delivery of nutrients to the cells. The fluid flow through the porous scaffold is modelled by Darcy’s law, and the delivery of nutrients to the cells is modelled by the advection–diffusion equation. A non-linear reaction diffusion system is used to model the cell growth. The growth of cells is modelled by logistic growth. COMSOL (a commercial finite element solver) is used to numerically solve the model. The results show that the distribution of cells and total cell number in the scaffold does not depend on the initial cell density but depend on the material permeability.