Abstract
A novel turn-on two-photon fluorescent probe NS-N2H4 was developed with the 2-benzothiazoleacetonitrile as a new recognition site for the detection of hydrazine (N2H4). The two-photon probe exhibited favorable properties including high selectivity, low cytotoxicity and almost 16-fold fluorescence enhancement in the presence of N2H4 in solution. The probe could be used to image hydrazine in the living cells. Notably, we also used the two-photon fluorescent probe to image hydrazine in the tissue imaging for the first time. Furthermore, by the way of probe-loaded TLC plate, we further monitored vapor of hydrazine. Therefore, the novel two-photon probe is expected to be employed to detect N2H4 in biosamples and environmental pollution and the new recognition site will be widely applied to construct fluorescent probes for the detection of N2H4.
Highlights
Hydrazine (N2H4) has been widely employed in space system as rocket propellant due to its special chemical properties including flammability and explosion[1]
There are some analytical methods for the detection of N2H4, which were exploited in the previous work, such as including chromatography-mass spectrometry, titrimetry and electro-chemical methods[7, 8]
To get insight into the proposed sensing process, we studied the reaction of NS-N2H4 with N2H4 by mass spectrometry
Summary
Hydrazine (N2H4) has been widely employed in space system as rocket propellant due to its special chemical properties including flammability and explosion[1]. According to its basic and reductive properties, hydrazine has been used as catalyst, corrosion inhibitor, and reducing agent in pharmaceutical, agricultural, and applied chemical industries[2,3,4] It is regarded as an important industrial pollutant to humans and animals with high toxicity, which could cause the lungs, livers, and kidneys cancerous[5]. It is highly significant to develop powerful means for the tracking and detection of N2H4 in living systems with high sensitivity and good selectivity. In the past few decades, organic fluorescent probes, which were regarded as the most powerful monitoring tools, have become an important tool used in biological studies with excellent merits including high sensitivity, good selectivity and real-time detection[9,10,11,12]. The two-photon probe is expected to be employed to detect N2H4 in biosamples and environmental pollution
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