294 INCOMPLETE THERMAL ABLATION INDUCES INCREASED PROLIFERATION OF RENAL CARCINOMA CELLS IN A MURINE MODEL

Abstract

You have accessJournal of UrologyKidney Cancer: Basic Research II1 Apr 2012294 INCOMPLETE THERMAL ABLATION INDUCES INCREASED PROLIFERATION OF RENAL CARCINOMA CELLS IN A MURINE MODEL Stephanie Kroeze, Harm van Melick, Maarten Nijkamp, Laura Kruijssen, Paul van Diest, Ruud Bosch, and Judith Jans Stephanie KroezeStephanie Kroeze Utrecht, Netherlands More articles by this author , Harm van MelickHarm van Melick Nieuwegein, Netherlands More articles by this author , Maarten NijkampMaarten Nijkamp Utrecht, Netherlands More articles by this author , Laura KruijssenLaura Kruijssen Utrecht, Netherlands More articles by this author , Paul van DiestPaul van Diest Utrecht, Netherlands More articles by this author , Ruud BoschRuud Bosch Utrecht, Netherlands More articles by this author , and Judith JansJudith Jans Utrecht, Netherlands More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.353AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Local recurrences of renal tumors occur frequently after radiofrequency ablation (RFA) and cryoablation (CA), and are observed more often compared to partial nephrectomy (PN). Although these high recurrence rates may be partly due to incomplete tumour ablation, stimulatory factors triggered by the thermal ablation could contribute to increased growth of surviving tumor cells. The aim of this study was to compare the presence of cell proliferation and stimulatory factors for growth and survival in renal tumors following RFA, CA and PN. METHODS Renca renal tumors were orthotopically transplanted under the renal capsule of mice (4-6 mice/group), and RFA, CA or PN was performed. Tumors were ablated incompletely (RFA and CA) or a part of the tumor was left behind (PN). At several time points (2h and 1, 3, 7, 14 days) hereafter, presence of cell proliferation (Ki67), hypoxia, inflammatory factors (CD45, F4/80) and the heat shock proteins (HSP70 and HSP90) was evaluated using immunohistochemistry. RESULTS Two hours following thermal ablation an increased growth of remaining tumor cells was observed, which peaked at 3 days and remained elevated for 2 weeks after thermal ablation. The hyperproliferation was significantly stronger after RFA compared to CA (p<0.05) and not present following PN. Hypoxia and HSPs were also increased from 2 hours, and up to 7 days after thermal ablation (p<0.0001), although HSP90 was only elevated in RFA treated tumors. The area of increased cell growth colocalized with the regions of hypoxia and HSP elevation. Inflammatory cells infiltrated at a later time point (24h) following thermal ablation, mainly within the necrotic areas following thermal ablation, and peaked at 1 week following ablation (p<0.05). All growth stimulatory factors were not or only slightly increased after PN CONCLUSIONS Following CA and particularly RFA, a significantly increased growth of remaining renal tumor cells is observed. This effect is not present following PN. Our study provides indications that the hypoxia and HSP70 may be responsible for the growth and survival of the remaining renal tumor cells. This underlines the importance of complete ablation for good oncological outcomes. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e119-e120 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Stephanie Kroeze Utrecht, Netherlands More articles by this author Harm van Melick Nieuwegein, Netherlands More articles by this author Maarten Nijkamp Utrecht, Netherlands More articles by this author Laura Kruijssen Utrecht, Netherlands More articles by this author Paul van Diest Utrecht, Netherlands More articles by this author Ruud Bosch Utrecht, Netherlands More articles by this author Judith Jans Utrecht, Netherlands More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...