294 Awardee Talk: Porcine Sperm Storage in the Oviduct Before Fertilization: A Sweet Spot

Abstract

Abstract Sperm storage in the female reproductive tract from sperm deposition until ovulation is conserved among a wide variety of animals allowing some asynchrony between mating and ovulation. Mammals, reptiles, birds, amphibians, and insects are among those that store sperm from a day or two to as long as a decade. We have studied the molecular basis of how sperm are retained and then released from the reservoirs in the female tract to fertilize oocytes, primarily in swine. Previous research by others implicated various oviduct glycans as receptors to retain sperm in the lower oviduct, the isthmus, but the complete structures of the glycans that bound sperm had not been identified. We used an array of hundreds of glycans to identify those that bound sperm and identified several glycans with sperm binding ability. Further inspection revealed that all sperm binding glycans contained at least one of two motifs, either a branched 6-sialylated structure or a Lewis X trisaccharide. Profiling all glycans produced by the isthmus showed that these motifs were abundant among the complex N-linked glycans of the isthmus. Further, they were found on the apical surface of the isthmic epithelium where they are available to bind sperm. These glycans suppressed the normal increase in intracellular free Ca2+ in sperm during capacitation and, when immobilized on beads, lengthened sperm lifespan, mimicking the effect of sperm of binding to the oviduct. Binding to oviduct glycans alters sperm metabolism in specific ways to prolong sperm viability. Sperm are released from immobilized glycans by progesterone, which induces hyperactivated motility, and by capacitation, which reduces the binding affinity of capacitated sperm for oviduct glycans. This information may be used to improve sperm lifespan in vivo or enhance sperm storage outside of the female tract.