Abstract
ABSTRACT Material and methods Blood from healthy female (n = 50) and breast cancer patients (n = 23, T1-2, N0-1, M0) were fractionated into plasma and cellular fractions, cell-surface-bound cirDNA (csbDNA) were eluted from cell surface with PBS/EDTA and trypsin solutions [1]. Total DNA was quantified with TaqMan PCR for LINE-1 repeats [2], methylated DNA of RARβ2 gene promoter was quantified with methylation-specific SYBR Green Real Time PCR [3]. Results Breast cancer patients had significantly higher mean concentration of methylated RARβ2 gene promoter in circulating DNA (cirDNA) and csbDNA fractions as compared to healthy controls: 192 vs. 132 pg/ml and 619 ng/ml vs. 413 pg/ml, respectively; P 1. Clin. Chem., 2005. V. 51. P. 1317-1319. 2. Anal. Biochem., 2011. V. 408. P. 354-356. 3. Eur. J. Cancer Prev., 2011. V. 20. P. 453-455. Disclosure All authors have declared no conflicts of interest. Despite of tumor location DNA from cancer cells was shown to got in circulating blood, thus DNA circulating in blood represent the valuable source of diagnostic material for non-invasive blood based tests for cancer.
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