292-POS

Abstract

Objectives The mechanisms that are involved in maintaining a human pregnancy to term, and the switches that lead to a normal labor and pregnancy outcome or indeed an adverse outcome such as miscarriage, preeclampsia, fetal growth restriction or preterm labor, are complex but the role of the placenta is crucial to them all. TLR family members are expressed differentially in a variety of cells and tissues. Toll-like receptors (TLR) are the principal signaling molecules through which mammals sense infection, so called innate immunity. Aims: The first aim of this study was to examine the spatial expression of TLRs in placentae obtained from women who delivered by caesarean section, and normal vaginal delivery, by defining precise sampling zones. The second aim was to determine the expression of TLRs in normal pregnancy with preeclampsia, both labor and non-labor. Methods Samples were obtained from 12 sites within each placenta: 4 equally spaced apart pieces were sampled from the inner, middle and outer placental zones. Non-labor, labor and preeclampsia were studied. TLRs gene expression was analyzed by RT-PCR using validated TaqMan® Gene Expression assays. Results There was a negotiable expression of TLR9 and 10 in the human placenta. There was a significant increase in TLR1 expression in the labor control compared to labor preeclampsia groups at the inner and middle sites ( p = 0.04, p = 0.002). TLR5 was significant increase in the non-labor group compared to labor group at the middle zones ( p = 0.004). No other differences were found. Conclusions TLRs may play a role in the physiology of labor and the pathology of pre-eclampsia. Disclosures A. Abdulsid: None. A. Fletcher: None. F. Lyall: None.