29] Purification of glutathione S-transferases by glutathione-affinity chromatography

Abstract

Publisher Summary The glutathione S-transferases from a variety of sources have been purified by the use of conventional chromatographic procedures, as well as by affinity chromatography. When affinity chromatography is used, it is generally necessary to apply additional separation procedures because most of the glutathione S -transferases contain several similar forms of the enzyme. This chapter describes the preparation of a glutathione-affinity chromatography column that has been successfully used for the purification of the glutathiones-transferases from the human liver. This procedure can serve as the initial step in the isolation of the family of glutathione S -transferases from a crude mixture, or it may be used as a final purification step after the several forms of the glutathione S -transferases are separated from one another.