Abstract
Publisher Summary Macrophage or monocyte cytotoxicity can be induced in various ways in vitro or in vivo. This cytotoxicity can be divided in cytostatic and cytolytic cytotoxicity. For the expression of both forms of cytotoxicity, direct contact of the target cell with the macrophages is required. The choice of the cytotoxicity assay depends on the type of cytotoxic activity that has to be measured. Cytolytic activities are generally detected by assays that measure the release of radiolabel from the target cells. Cytostatic activities can be detected by assays that measure the incorporation of radiolabels in DNA such as [ 3 H]thymidine and [ 125 I]iododeoxyuridine. The overall effect of cytostatic and cytolytic macrophage activity can be studied by visual cell counting of nonadherent target cells. Cytotoxicity of murine peritoneal macrophages is usually tested in confluent macrophage monolayers in flat-bottom wells. Human monocytes are relatively weakly adherent and cannot be washed as vigorously as murine macrophages.
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