28457 Visualization of penetration of topical antifungal drug substances through mycosis-infected nails by matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI)

Abstract

Introduction: Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) is a mass spectrometry based technique which can be applied for compound-specific imaging of pharmaceuticals in tissues samples. MALDI-MSI technology is widely used to visualize penetration and distribution profile through different tissues but has never been used with nail tissue, especially when infected with onychomycosis. This study used MALDI-MSI technology to visualize distribution profile and penetration into ex vivo human mycosis-infected toenails of three antifungal active ingredients amorolfine, ciclopirox and naftifine contained in topical onychomycosis nail treatment preparations.