Abstract
Publisher Summary The hemolymph of the beetle Allomyrina dichotoma contains two lectins (Allo A-I and Allo A-II) that agglutinate human erythrocytes. The hemagglutination is inhibited by a variety of β-galactoside derivatives. The amount of lectins markedly increases at the stage of third instar larva of the beetle. Investigation of the behavior of various oligosaccharides on an immobilized Allo A-II column revealed that the column is very useful for the analysis of sialylated oligosaccharides. A simple purification method for Allo A-II, the properties of the lectin, and the binding specificity of a column containing immobilized Allo A-II are described in this chapter . a column containing immobilized Allo A-II can be used as an effective tool to separate isomeric sialooligosaccharides. It has been found that a column containing immobilized Allo A-I gives the same fractionation patterns of oligosaccharides as described here for an Allo A-II column. Separation of the two isolectins is consequently unnecessary for the purpose of fractionating oligosaccharides.
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