279P - Genetic Polymorphism in the Mir-196A As a Prognostic Biomarker for Early Breast Cancer

Abstract

ABSTRACT Aim: As microRNAs (miRNA) may play important roles in tumorigenesis by regulating the expression of proto-oncogenes or tumor suppressor genes, the present study analyzed SNPs located in miRNA and miRNA-binding sites of various genes and their impact on the prognosis for 452 patients with early breast cancer. Methods: Three SNPs (rs3746444, rs11614913, and rs1044129) were selected using in silico analysis and genotyped using the Sequenom MassARRAY. Results: The median age of all the patients was 48 years, and 283 (62.6%) had ER/PR-positive, 86 (19.0 %) had HER2-overexpressed, and 77 (17.0 %) had triple-negative EBC. During the median follow-up of 6.9 years, 67 (14.8%) relapses and 55 (12.2%) deaths occurred. Among the 3 polymorphisms miR-196a rs11614913T > C was significantly associated with DFS and distant DFS (DDFS) when adjusted for clinical and pathological parameters. In particular, the prognostic impact of rs11614913 was limited to the hormone receptor- expressed subtype, where the patients bearing the CC genotype showed worse survival in terms of RFS and DDFS compared with the patients with the TT or TC genotype as a recessive model (hazard ratio [HR] = 2.610 and P = 0.003 for PFS; HR = 2.730 and P = 0.013 for DDFS). Conclusions: The current study provides evidence that the miR-196a rs11614913T > C polymorphism is a possible prognostic biomarker for patients with hormone receptor-expressed early breast cancer. Disclosure: All authors have declared no conflicts of interest.