279 Instrument-free single-cell resolution of transcriptome in human skin

Abstract

Single-cell RNA sequencing is a promising and robust technique that has been widely applied in the field of dermatology to study the transcriptional profile of each cell at very high resolution. Here we tested the compatibility of the novel instrument-free single-cell RNA-sequencing kit, recently developed by Scipio bioscience, on human skin cells. Human skin punch biopsy was first mechanically treated for a separation into dermis and epidermis fractions, then each fraction was separately processed for cell dissociation.