279 4.EFFECT OF LONG-TERM PRAZOSIN AND YOHIMBINE ADMINISTRATION ON C-FOS EXPRESSION IN SPINAL NEURONS: INHIBITORY EFFECT OF ALPHA-1 AND -2 ADRENOCEPTORS ON AFFERENTS FROM THE LOWER URINARY TRACT

Abstract

You have accessJournal of UrologyBladder and Urethra: Anatomy, Physiology and Pharmacology (I)1 Apr 2013279 4.EFFECT OF LONG-TERM PRAZOSIN AND YOHIMBINE ADMINISTRATION ON C-FOS EXPRESSION IN SPINAL NEURONS: INHIBITORY EFFECT OF ALPHA-1 AND -2 ADRENOCEPTORS ON AFFERENTS FROM THE LOWER URINARY TRACT Nobuhiro Haga, Yoshiyuki Kojima, Masanori Nomiya, Koji Sagawa, Ken Kumagai, Souichiro Ogawa, and Jyunya Hata Nobuhiro HagaNobuhiro Haga Fukushima, Macedonia More articles by this author , Yoshiyuki KojimaYoshiyuki Kojima Fukushima, Macedonia More articles by this author , Masanori NomiyaMasanori Nomiya Fukushima, Macedonia More articles by this author , Koji SagawaKoji Sagawa Fukushima, Macedonia More articles by this author , Ken KumagaiKen Kumagai Fukushima, Macedonia More articles by this author , Souichiro OgawaSouichiro Ogawa Fukushima, Macedonia More articles by this author , and Jyunya HataJyunya Hata Fukushima, Macedonia More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1663AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES To investigate effects of long-term administration of α1-adrenoceptor antagonist prazosin and αéQ-AR antagonist yohimbine on afferent inputs from the lower urinary tract by evaluating c-Fos expression in the spinal cord. METHODS Prazosin or yohimbine was administered for 4 weeks in rats using an osmotic pump. Effects of these agents on urodynamic parameters were determined by continuous cystometry in conscious rats. After cystometry, c-Fos expression in the dorsal horn of the L6 spinal cord was measured by immunohistochemistry. RESULTS Administration of prazosin (0.12 mg/kg/day) or yohimbine (0.10mg/kg/day) significantly increased micturition interval and bladder capacity, but did not affect micturition pressure and residual urine volume (table). Numbers of c-Fos-positive neurons in the dorsal horn were significantly lower in rats that received prazosin than in controls (figure).Yohimbine reduced the number of c-Fos-positive neurons in the part of dorsal horn. CONCLUSIONS Long-term administration of prazosin and yohimbine at clinically recommended doses can exert inhibitory effects on afferent pathways from the lower urinary tract during the storage phase. These reductions of the afferent input result in the increased bladder capacity and increased micturition interval. micturition parameters control prazosin yohimbine Micturition pressure (cmH2O) 42±3 54±3 52±11 Micturition interval (min) 4.33±0.13 6.38 ±0.18* 6.80 ±0.9* Cystometric capacity (ml) 0.72±0.22 1.06 ±0.03* 1.13±0.15* Residual volume (ml) 0 0.05±0.07 0.03±0.03 Micturition parameters in control rats (n=10), prazosin (0.12 mg/kg/day)-administered rats (n=10) and yohimbine (0.10 mg/kg/day)-administered rats (n=10). *p < 0.01 vs. Control group. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e114 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Nobuhiro Haga Fukushima, Macedonia More articles by this author Yoshiyuki Kojima Fukushima, Macedonia More articles by this author Masanori Nomiya Fukushima, Macedonia More articles by this author Koji Sagawa Fukushima, Macedonia More articles by this author Ken Kumagai Fukushima, Macedonia More articles by this author Souichiro Ogawa Fukushima, Macedonia More articles by this author Jyunya Hata Fukushima, Macedonia More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...