#278 : Relationship Between Level Expression of miRNA-191 and miRNA-548c-3p in Embryo Culture Medium and Embryo Chromosomal Status

Abstract

Background: microRNA (miRNA) is expressed by the human embryos and shows the difference between euploid and aneuploid embryos. miRNA which is secreted in culture media is expected to be a biomarker for embryo chromosomal status testing. This research aims to analyse the relationship between expression level of miRNA-191 and miRNA-548c-3p in embryo culture media and embryo chromosomal status. Methods: This cross-sectional research was done on 30 embryos from 12 patients aged 28-40 years old who underwent an in vitro fertilization (IVF) program in three clinics. Embryos were cultured until blastocyst stage. PGT-A was done after blastocyst biopsy on day 5 or 6, and chromosomal analysis was done using the NGS method. The expression levels of miRNA-191 and miRNA-548c-3p were analysed from culture media sample of the embryo using qPCR method. Then the relationship between miRNA-191 and miRNA-548c-3p expression level with embryo chromosomal status was analysed. Results: There is no significant difference in subjects’ characteristics between the euploid and aneuploid embryo groups (p>0.05) [Table 1]. There is no significant difference in the expression level of miRNA-191 of the euploid and aneuploid embryo groups. (Median quantification 8.69 vs 1.064 with p=0.351) [Table 2]. There is no significant difference in the expression level of miRNA-548c-3p of the euploid and aneuploid embryo groups. (Median quantification 9.06 vs 6.635 with p=0.709) [Table 3]. Conclusions: miRNA-191 expression level and miRNA-548c-3p expression level has no relationship with embryo chromosomal status. The level expression of miRNA-191 and miRNA-548c-3p in embryo culture has not been a good biomarker candidate for embryo chromosomal status.